Instructor:                                                                 

                Office:                              Campus                            

Office Hours:                                                             

Phone:                         E-Mail:                                   

 

SYLLABUS

MIDLANDS TECHNICAL COLLEGE

SCIENCE DEPARTMENT

Fall Semester 2002

 

COURSE TITLE:  MICROBIOLOGY                                                                                    COURSE NUMBER:  BIO 225

 

CREDIT HOURS: 4.0                                                    LECTURE: 3.0                              LABORATORY: 1.0

CONTACT HOURS: 6.0                                                LECTURE: 3.0                              LABORATORY: 3.0

 

COURSE DESCRIPTION:

 

This is a detailed study of microbiology as it relates to infection and the disease processes of the body.  Topics include immunity, epidemiology, medically important microorganisms, and diagnostic procedures for identification.  Application to clinical health will be emphasized.

 

COURSE OBJECTIVE:

 

The objective of this course is to assist the student in gaining a working knowledge of the basic principles and concepts of microbiology and infectious disease and the application of these concepts to human disease.

 

LEARNING OBJECTIVES:

 

Learning objectives are available from your instructor.

 

TEXT:

 

Microbiology, An Introduction, Totorta, 7th, 2001.

MTC Microbiology Laboratory Manual, Yurko, 1st ed.

Photographic Atlas of Microbiology Lab, Leboffe, 2nd., 99, Morton

 

OPTIONAL MATERIAL:

 

Lecture notes or other material designated by instructor other than required texts listed on this course syllabus are considered optional.

 

PRE-REQUISITES AND CO-REQUISITES:

 

Prerequisite: Bio. 101 or Bio. 211

 

COURSE FIELD TRIPS (IF ANY): None

 

DISABILITIES:

 

Students with disabilities requiring in-class accommodations should call the Counseling/Disabilities Resource Center at 738-7637 (Beltline) or 822-3505 (Airport).

 

ATTENDANCE POLICY:

 

Students will be allowed to miss twice the number of times a lecture or laboratory section meets per week. 

 

If the lecture meets 3 times per week, 6 absences are allowed.

If the lecture meets 2 times per week, 4 absences are allowed.

If the laboratory meets 2 times per week, 4 absences are allowed.

 

If the student misses more than 10 minutes of class by either arriving late or leaving early, then the student will be counted as

absent, missing fewer than 10 minutes is a tardy.  Three tardies count as one absence.

 

Students adding courses after classes begin are responsible for work covered from the first day of classes.  All classes missed are counted as absences.

 

When a student exceeds the maximum allowable absences, a grade of "W" will be assigned if the student is passing the course or "WF" if the student is failing after midterm.  A "WF" is calculated into the grade point average as an "F.”

 

GRADING:

 

GRADE CALCULATIONS FOR BIO 225

 

LECTURE GRADE = 75% of the final grade

 

Exam 1:              

Exam 2:              

Exam 3:              

Exam 4:              

              Comprehensive Final Exam:             

Sum:                   

 

Lecture average = (Sum)        / 5 (# of lecture exams) =             

Lecture points = (lecture average)                   X 0.75 =                  

       

(Each exam counts 15%)

 

 

LABORATORY GRADE = 25% of the final grade

 

Laboratory Practical I (Midterm) =                            (Possible 300 points)

Laboratory Practical II (Final) =                                (Possible 300 points)

Identification of a Bacterial Unknown =                    (Possible 150 points)

Laboratory Technique =                                            (Possible 100 points)

Organism Reports =                                                   (Possible   50 points)

Case studies =                                                            (Possible   50 points)

Internet exercise =                                                     (Possible   50 points)

Total points =                                                             (Possible 1000 points)  

 

Lab grade calculations are based on 1000 points.

 

Lab average = Total points            /1000 =                    

Lab points = (lab average)            X 0.25 =                 

 

(Lecture points)             (Lab points) =                (Course Average)

 

If course average is:                                                Final Course Grade is:

 

90-100                                                                    A

80-89                                                                      B

70-79                                                                      C

60-69                                                                      D

 <60                                                                        F

Withdrawal before midterm                                                W

Withdrawal PASSING after midterm                                   W

Withdrawal FAILING (<60) after midterm                            WF

 

All course work not completed in the course will be graded as “0" and counted in the final average.  No grades may be dropped in this course.  No extra credit may be given in this course unless it is equally available to all sections of the course.

 

BIOLOGY 225 MICROBIOLOGY

 

LABORATORY GRADING

 

I. LABORATORY TECHNIQUE                                                                      

 

A.  POUR PLATE                                                                                             10 POINTS

B.  ASEPTIC TRANSFER                                                                                 10 POINTS

C.  STREAKING FOR ISOLATI                                                                         20 POINTS

D.  GRAM STAIN PRACTICAL                                                                          40 POINTS

               E.  MISC. EXERCISES                                                                                      20 POINTS

                                                                                                             TOTAL             100 POINTS

                                                                                                                                                                                               

II. LITERATURE AND INTREPRETATION                                                                                   

 

            A. ORGANISM REPORTS                                                                                 50 POINTS

                Each student will give 2 reports on organisms that will be assigned by the instructor from a

                selected list.  Reports will be both written and oral.  A one-page form will be supplied for the

                written report.

            

            B. CASE STUDIES                                                                                           50 POINTS

            

            C. INTERNET EXERCISE                                                                                  50 POINTS

                                                                                                             TOTAL          150 POINTS

 

III.     LABORATORY PRACTICALS                                                              

 

A. MIDTERM                                                                                                     300 POINTS

B. FINAL                                                                                                           300 POINTS

            All laboratory practicals are departmental.  Lab           TOTAL            600 POINTS

            instructors may not drop questions from

            practicals, may not curve or scale practicals, and

            may not give any bonus credit questions.

 

IV. IDENTIFICATION OF A BACTERIAL UNKNOWN                                   

 

A.  MORPHOLOGY AND GRAM STAIN                                                             50 POINTS

B.  IDENTIFICATION OF UNKNOWN                                                                50 POINTS

C.  BIOCHEMICAL TESTS                                                                                50 POINTS

                                                                                                            TOTAL             150 POINTS

 

 

                          TOTAL POSSIBLE POINTS: 1000

 

 

LAB WORK IN THIS COURSE CANNOT BE MADE UP.

STUDENTS MAY NOT ATTEND LABS OTHER THAN THEIR OWN.

 

SPECIAL NOTE

 

All written work done by students in partial fulfillment of the course requirement must be typed.  This includes organism reports and class assignments and/or case study reports.  Instructors may not accept hand written reports.  All MTC students have access to our computer labs and it is required that all students be computer literate.

 

Unknown morphology and unknown report forms are expected to be hand written.  However, how well the information is reported by the student may be considered in grading.  Neatness and completeness do count.

 

Work not turned in on time will be accessed a 10% point penalty per incidence.

Students may be assessed a point penalty for each violation (up to 5 points for each violation) of the performance criteria such as improper disposal of infectious material, inability to properly light the Bunsen burner, improper storage and use of the microscope, and failure to wear lab coats at all lab work sessions.

 

TENTATIVE WEEKLY SCHEDULE

TENTATIVE WEEKLY SCHEDULE

 

 

WEEK                                                  LECTURE TOPIC                              CHAPTER

 

1                                                   Introduction                                                                               1

                                                    Chemical Principles (review)                                                   2

                                                    Microscopy (covered in lab)                                                     3

 

2                                                  Prokaryotic Cell                                                                           4

                                                    Microbial Metabolism                                                                           5

 

3                                                  Microbial Growth                                                                         6

                                                    Control of Microbial Growth                                                            7

 

4                                                  EXAMINATION I                                                                           1-7

                                                    Microbial Genetics                                                                      8

                                                    Biotechnology                                                                                          9

 

5                                                  Classification of Microorganisms                                           10

                                                    Bacteria                                                                                           11

 

6                                                  Fungi and Algae                                                                          12

                                                   Protozoans, and Helminths                                                     12

 

7                                                  Viruses                                                                            13          

                                                    EXAMINATION II                                                                            8-13

 

8                                                  Principles of Diseases and Epidemiology                           14

                                                   Mechanisms of Pathogenicity                                                 15

 

9                                                 Nonspecific Defenses                                                               16

                                                   The Immune Response                                              17

 

10                                                Applications of Immunology                                                    18

                                                    Immune Disorders                                                                           19

 

11                                                 Antimicrobial Drugs                                                                   20

                                                    EXAMINATION III                                                                           14-20              

 

12                                                            

                                           Diseases of the Skin and Eyes                                                21

Diseases of the Nervous System                                             22              

Diseases of the Cardiovascular System

and Lymphatic Systems                                                            23

 

13                                                        Diseases of the Cardiovascular System

and Lymphatic Systems                                                            23

Diseases of the Respiratory System                                             24

Diseases of the Digestive System                                             25

 

14                                                                                                                                                                                                              Diseases of the Urinary Tract                                                  26

Sexually Transmitted Diseases                                             26

Examination IV                                                                               21-26

TBA                                                                                                 DEPARTMENTAL CUMULATIVE

FINAL EXAMINATION                                                                           1-26

 
 
 
TENTATIVE WEEKLY LABORATORY SCHEDULE

 

Date

 

Week

Topic

Module

MW

TR

 

 

 

 

 

 

 

 

19-Aug

20-Aug

1

Introduction to the microbiology lab

 

 

 

 

Microscopy

1

22-Aug

22-Aug

 

Microscopy

1

26-Aug

27-Aug

2

Oil immersion

1

28-Aug

29-Aug

 

Oil immersion finish

1

 

 

 

Observation of a wet mount (begin)

2

2-Sep

3-Sep

3

No lab

 

4-Sep

5-Sep

 

Observation of a wet mount (finish)

2

 

 

 

 pH Exercise

Handout

9-Sep

10-Sep

3/4

Preparation of a pour plate (begin)

3

 

 

 

Ubiquity of microorganisms (begin)

4

11-Sep

12-Sep

 

Preparation of a pour plate (finish)

3

 

 

 

Ubiquity of microorganisms (finish)

4

 

 

 

Cultural characteristics of bacteria

9

 

 

 

 

 

16-Sep

17-Sep

4/5

Aseptic transfer of microbes (begin)

5

 

 

 

Streaking for isolation (begin)

6

18-Sep

19-Sep

 

Aseptic transfer of microbes (finish)

5

 

 

 

Streaking for isolation (finish)

6

 

 

 

Cultural characteristics of bacteria

9

 

 

 

The Gram stain

7

23-Sep

24-Sep

5/6

The Gram stain

7

 

 

 

Special staining techniques (begin)

8

25-Sep

26-Sep

 

Gram stain practical

 

 

 

 

Special staining techniques (finish)

8

30-Sep

1-Oct

6/7

Exoenzymes (begin)

10

2-Oct

3-Oct

 

Exoenzymes (finish)

10

 

 

 

pH Indicator Molecules

Handout

 

 

 

Internet Studies

Handout

 

 

 

Review for Practical

 

7-Oct

8-Oct

8

No labs

 

9-Oct

10-Oct

 

Mid-term laboratory practical

1-7

14-Oct

15-Oct

8/9

Selective & differential media (begin)

11

16-Oct

17-Oct

 

Selective & differential media (finish)

11

 

 

 

Protein Concentration

Handout

21-Oct

22-Oct

9/10

Further tests for gram negative microorganisms (begin)

12

23-Oct

24-Oct

 

Further tests for gram negative microorganisms (finish)

12

 

 

 

DNA Extraction

Handout

 

 

 

Electrophoresis

Handout

28-Oct

29-Oct

10/11

Tests for gram positive cocci (begin)

13

 

 

 

Miscellaneous chemical tests (begin)

14

30-Oct

31-Oct

 

Tests for gram positive cocci (finish)

13

 

 

 

Miscellaneous chemical tests (finish)

14

 

 

 

Unknown Assigned

Handout

4-Nov

5-Nov

11/12

No labs

 

6-Nov

7-Nov

 

Unknown

Handout

11-Nov

12-Nov

12/13

Effects of antibiotics (begin)

15

 

 

 

Unknown

Handout

13-Nov

14-Nov

 

Effects of antibiotics (finish)

15

 

 

 

Unknown

Handout

18-Nov

19-Nov

13/14

Unknown

Handout

20-Nov

21-Nov

 

Unknown

Handout

 

 

 

Bacterial transformation

 

25-Nov

26-Nov

14

Unknown due (no exceptions)

Handout

 

 

 

Review for final lab practical

 

27-Nov

28-Nov

 

No labs

 

 

 

14

 

 

2-Dec

3-Dec

 

Final lab practical

10-15

             

* All students, faculty and staff must understand that food and drinks cannot be allowed in lab at any time.  This includes packaged items in book bags.  This is an OSHA violation and can result in significant fines for the school.  It is also highly dangerous.

 

Assigned Reading for Lecture- Atlas

 

Week

Topic

 

7

Precipitation Reactions

106-107

7

Agglutination Reactions

108-109

7

Enzyme-linked Immunosorbent Assay (ELISA)

110-111

7

Florescent Antibody (FA) Technique

112

7

Western Blot Technique

113-114

5

Viruses

115-116

5

HIV

117

5
Cytopathic Effects

118

4

Bacterial Pathogens

131-142

4

Survey of Fungi

143-150

4

Parasitic Protozoans & Helminths

151-175

2

Glycolysis, Fermentation, Krebs Cycle

-177-182

 

 

 

 

LABORATORY PERFORMANCE CRITERIA

 

1.    Lab coats worn during lab work sessions.

 

2.    Microscopes handled, used and stored in a correct manner.

 

3.    Lab tables kept clean and disinfected before and after each lab session.

 

4.    Proficiency in focusing the microscope at all levels.

 

5.    Proficiency in lighting and using a Bunsen burner.

 

6.    Aseptic techniques used when working with cultures.

 

7.    Listening to instructors’ directions and following these directions in the                                 performance of laboratory exercises.

 

8.    Carrying out procedures in a proper, safe and correct manner.

 

9.    Disposal of material in the appropriate manner.

 

10.  Observance of safe laboratory practices.

 

11.  Completion of each laboratory assignment.

 

12.   Working well with partners — Each student should do their part in team activities.

 

 

ASSIGNMENTS AND MISSED TESTS:

 

Students who are absent from a class are responsible for all of the work that is done during that class period and for all assignments made during the missed class period.

 

One (1) missed lecture exam may be made up if a legitimate excuse is presented to your lecture instructor. All make-ups will be at the convenience of the lecture instructor. Missed laboratory work cannot be made up.  This includes quizzes.

 

The Science Department Chair, Coordinators, and faculty are here to help you.  If you are having any problems in your classes, please contact the people who can help you.  If we don’t know you are having problems, we can’t help you.  The Department Chair @ 822-3443; Regina Hoffman  is Airport Coordinator @ 822-3416; Bert Knesel is Beltline Coordinator @738-7660.

 


 

CHANGES:

 

The instructor reserves the right to make changes in the lecture and laboratory schedule as deemed necessary. 

Additional policies for the Department are available in the Science Department’s Student Policy and Procedures Handbook, in the Academic Center, Room 368; Robinson 106; Airport and Beltline Libraries, and Lindau Engineering Tech. Building, Room 421.

 

LABORATORY POLICIES AND SAFETY PRECAUTIONS

 

The laboratory experience is designed to supplement and complement material covered in lecture, and is an integral part of the course.  Material may be presented in greater detail in lab than in lecture.  The laboratory experience may include dissections, films, demonstrations, experiments, workbook exercises, research and writing assignments, and field trips.  The day, time and location of approved field trips will be indicated in the course syllabus.  Approved field trips will be held during regularly scheduled lab times.  Students will provide their own transportation.  The laboratory instructors will give a sufficient amount of information and guidance to allow students to complete each lab, but success in lab primarily involves the individual effort of each student.  To maximize learning and to minimize the risk of accidents or injury, the following policies and rules will be observed in science labs:

 

 1.            Laboratory exercises must be completed during the assigned lab period unless otherwise designated by your instructor. 

 

 2.            Students are expected to read laboratory exercises before coming to class.

 

 3.        Do not disturb any equipment or demonstrations that have been set up, until your instructor has told you how to proceed with the assignment.

 

 4.        Apply yourself in lab.  The exercises are designed to reinforce and expand upon material presented in the lecture. Follow directions carefully.

 5.        No more than four people can work at any laboratory bench at the same time.

 

 6.        Do not hesitate to ask your lab instructor for clarification of any instructions you do not understand. However, do not expect your lab instructor to simply give you answers to information you should be obtaining through the completion of the various lab exercises.

 

 7.        Bring all necessary materials to lab, including your text.

 

 8.        Do not bring any food or drinks into any science lab.

 

9.              Know where the eyewash station and shower are located in each lab.  Report injuries immediately to your lab instructor.

 

10.            Protective eyewear is not needed in microbiology, but lab coats are ESSENTIAL.

 

11.       At the end of each lab, leave your work space in good order by discarding waste materials, cleaning and disinfecting your lab table, returning all materials used to their proper place, and sliding your chair back under the table.  Wash your hands.

 

12.             No visitors are allowed in lab or lecture.

 

13.             Neglect of proper safety procedures will result in the student being required to leave the lab and will be required to meet with the department chair before re-admittance.

MIDLANDS TECHNICAL COLLEGE SCIENCE DEPARTMENT

CODE OF CONDUCT

 

 

Student rights and responsibilities are outlined in the Student Handbook. We are extremely proud of the quality of students in the Science Department, however, there have been occasions where disciplinary action is necessary to prevent disruptive and dishonest behavior.  The following items are specific violations and consequences supported by the Science Department. Your instructor will circulate a form for your signature stating that you understand the Science Department Course Syllabus, which includes this document. 

 

1.      Any student who exhibits behavior that is disruptive to the learning process such as talking, discourtesy to faculty or fellow students to include obscene language or gestures, or uncooperative actions will be asked to leave the classroom.  The student will be counted absent for this class.  Depending upon the nature of the offense or if it occurs during an exam the instructor may require that the student see the Science Coordinator, Chair of the Science Department, or the Director of Campus Life before returning to class.  Campus Security will be called for any threatening or violent behavior.   

 

2.             Beepers, cell phones, personal stereos, and similar devices are not permitted in class.              Permission must be obtained from the Science Coordinator or Instructor for students             who are emergency personnel or where there are extenuating circumstances.  Campus             Security can locate a student and will interrupt a class if there is a situation that needs             immediate attention.

 

3.                Any student proven to have engaged in academic dishonesty will be given a grade of zero on the exam or assignment.   This includes, but is not limited to, giving or receiving information during an exam, use of unauthorized materials during an exam or assignment, plagiarism, or changing answers after a grade has been assigned.  An instructor must have reasonable proof that dishonesty has occurred.  Until an incident is verified, the student will be assigned a grade of “I” for the work.  Witnesses of cheating should report this immediately to the instructor.  The grade will be discussed confidentially with the student.  If the student denies that academic dishonesty occurred, the Chair of the Science Department or Science Coordinator will meet with the instructor and student.  The instructor will be supported if departmental guidelines for handling cheating incidences were followed.  However, the student is referred to the Student Handbook for the policy on filing a grievance.  In any incident involving academic dishonesty, a report will be filed with the Director of Campus Life. 

 

4.      Students with complaints about instructors should follow the appropriate chain of       command as outlined in the "Science Department Conflict Resolution" form.  A form can be obtained from the Science Department.  Signatures must be obtained at each level before the complaint will be validated.   There may be some circumstances where       the first contact is with the Science Coordinator who will discuss the problem with the       instructor.   All efforts possible will be made to resolve conflicts internally.  However students should remember that matters can also be handled through the Academic       Appeal/Grievance process detailed in the Student Handbook.