Instructor: Office Hours: __________________Phone: E-Mail
MIDLANDS TECHNICAL COLLEGE
SCIENCE DEPARTMENT
COURSE TITLE: MICROBIOLOGY COURSE NUMBER: BIO 225
CREDIT HOURS: 4.0 LECTURE: 3.0 LABORATORY: 1.0
CONTACT HOURS: 6.0 LECTURE: 3.0 LABORATORY: 3.0
COURSE DESCRIPTION:
This is a detailed study of microbiology as it relates to infection and the disease processes of the body. Topics include immunity, epidemiology, medically important microorganisms, and diagnostic procedures for identification. Application to clinical health will be emphasized.
COURSE OBJECTIVE:
The objective of this course is to assist the student in gaining a working knowledge of the basic principles and concepts of microbiology and infectious disease and the application of these concepts to human disease.
LEARNING OBJECTIVES:
Learning objectives are available from your instructor.
TEXT:
Microbiology, An Introduction, Totorta, 7th, 2001.
MTC Microbiology Laboratory Manual, Yurko, 1st ed.
Photographic Atlas of Microbiology Lab, Leboffe, 2nd., 99, Morton
OPTIONAL MATERIAL:
Lecture notes or other material designated by instructor other than required texts listed on this course syllabus are considered optional.
PRE-REQUISITES AND CO-REQUISITES:
Prerequisite: Bio. 101 or Bio. 211
COURSE FIELD TRIPS (IF ANY): None
DISABILITIES:
Students with disabilities requiring in-class accommodations should call the Counseling/Disabilities Resource Center at 738-7637 (Beltline) or 822-3505 (Airport).
ATTENDANCE POLICY:
Students will be allowed to miss twice the number of times a lecture or laboratory section meets per week.
If the lecture meets 3 times per week, 6 absences are allowed.
If the lecture meets 2 times per week, 4 absences are allowed.
If the laboratory meets 2 times per week, 4 absences are allowed.
If the student misses more than 10 minutes of class by either arriving late or leaving early, then the student will be counted as absent, missing fewer than 10 minutes is a tardy. Three tardies count as one absence.
Students adding courses after classes begin are responsible for work covered from the first day of classes. All classes missed are counted as absences.
When a student exceeds the maximum allowable absences, a grade of "W" will be assigned if the student is passing the course or "WF" if the student is failing after midterm. A "WF" is calculated into the grade point average as an "F.”
GRADING:
GRADE CALCULATIONS FOR BIO 225
LECTURE GRADE = 75% of the final grade
Exam 1:
Exam 2:
Exam 3:
Exam 4:
Comprehensive Final Exam:
Sum:
Lecture average = (Sum) / 5 (# of lecture exams) =
Lecture points = (lecture average) X 0.75 =
(Each exam counts 15%)
LABORATORY GRADE = 25% of the final grade
Laboratory Practical I (Midterm) = (Possible 325 points)
Laboratory Practical II (Final) = (Possible 325 points)
Identification of a Bacterial Unknown = (Possible 125 points)
Laboratory Technique = (Possible 100 points)
Organism Reports = (Possible 50 points)
Case studies = (Possible 50 points)
Internet exercise = (Possible 25 points)
Total points = (Possible 1000 points)
Lab grade calculations are based on 1000 points.
Lab average = Total points /1000 =
Lab points = (lab average) X 0.25 =
(Lecture points) (Lab points) = (Course Average)
If course average is: Final Course Grade is:
| 90-100 | A |
| 80-89 | B |
| 70-79 | C |
| 60-69 | D |
| <60 | F |
| Withdrawal before midterm | W |
| Withdrawal PASSING after midterm | W |
| Withdrawal FAILING (<60) after midterm | WF |
All course work not completed in the course will be graded as “0" and counted in the final average. No grades may be dropped in this course. No extra credit may be given in this course unless it is equally available to all sections of the course.
BIOLOGY 225 MICROBIOLOGY
LABORATORY GRADING
|
A. POUR PLATE B. ASEPTIC TRANSFER C.
STREAKING FOR ISOLATION E. MISC. EXERCISES
TOTAL
|
10 POINTS 10 POINTS 20 POINTS 40 POINTS 20 POINTS
100 POINTS
|
II. LITERATURE AND INTREPRETATION
|
A. Organism Reports Each student will give 2 reports on organisms that will be assigned by the instructor from a selected list. Reports will be both written and oral. A one-page form will be supplied for the written report. |
50 Points
|
| B. Case Studies | 50 Points |
| C. Internet Exercise | 25 Points |
| Total | 125 Points |
III. LABORATORY PRACTICALS
|
A. Midterm B. Final All laboratory practicals are departmental. Lab instructors may not drop questions from practicals, may not curve or scale practicals, and may not give any bonus credit questions.
Total
|
325 Points 325 Points
650 Points
|
IV. IDENTIFICATION OF A BACTERIAL UNKNOWN
| A. Morphology and Gram Stain | 25 Points |
| B. Identification of Unknown | 100 Points |
| Total | 125 Points |
| Total Possible Points: | 1000 |
LAB WORK IN THIS COURSE CANNOT BE MADE UP.
STUDENTS MAY NOT ATTEND LABS OTHER THAN THEIR OWN.
SPECIAL NOTE
All written work done by students in partial fulfillment of the course requirement must be typed. This includes organism reports and class assignments and/or case study reports. Instructors may not accept hand written reports. All MTC students have access to our computer labs and it is required that all students be computer literate.
Unknown morphology and unknown report forms are expected to be hand written. However, how well the information is reported by the student may be considered in grading. Neatness and completeness do count.
Work not turned in on time will be accessed a 10% point penalty per incidence.
Students may be assessed a point penalty for each violation (up to 5 points for each violation) of the performance criteria such as improper disposal of infectious material, inability to properly light the Bunsen burner, improper storage and use of the microscope, and failure to wear lab coats at all lab work sessions.
TENTATIVE WEEKLY SCHEDULE
MICROBIOLOGY - BIO 225
|
WEEK |
LECTURE TOPIC |
CHAPTER |
|
1 |
Introduction Chemical Principles (review) Microscopy (covered in lab) Prokaryotic Cell |
1 2 3 4 |
|
2 |
Microbial Metabolism Microbial Growth Control of Microbial Growth |
5 6 7 |
|
3 |
EXAMINATION 1 Microbial Genetics Biotechnology |
1-7 8 9 |
|
4 |
Classification of Microorganisms Bacteria Fungi and Algae |
10 11 12 |
|
5 |
Protozoans and Helminths Viruses Principles of Diseases and Epidemiology |
12 13 14 |
|
6 |
EXAMINATION II Mechanisms of Pathogenicity Nonspecific Defenses |
8-13 15 16 |
|
7 |
The Immune Response Applications of Immunology Immune Disorders |
17 18 19 |
|
8 |
Antimicrobial Drugs EXAMINATION III Diseases of the Skin and Eyes |
20 14-20 21 |
|
9 |
Diseases of the Nervous System Diseases of the Cardiovascular System And Lymphatic Systems Diseases of the Respiratory System |
22 23
24 |
|
10 |
Diseases of the Digestive System Diseases of the urinary Tract Sexually Transmitted Diseases EXAMINATION IV |
25 26 26 21-26 |
|
TBA |
DEPARTMENTAL CUMULATIVE FINAL EXAM |
1-26 |
Microbiology Tentative Weekly Laboratory
|
Date |
|
Week |
Topic |
Module |
|
MW |
TTH |
|
|
|
|
|
|
|
|
|
|
26-May |
27-May |
1 |
Introduction to the microbiology lab |
|
|
|
|
|
Microscopy |
1 |
|
28-May |
29-May |
|
Microscopy |
1 |
|
|
|
|
Oil immersion |
|
|
02-June |
03-June |
2 |
Observation of a wet mount |
2 |
|
|
|
|
Preparation of a pour plate (begin) |
3 |
|
|
|
|
Ubiquity of microorganisms (begin) |
4 |
|
04-June |
05-June |
|
Preparation of a pour plate (finish) |
3 |
|
|
|
|
Ubiquity of microorganisms (finish) |
4 |
|
|
|
|
Cultural characteristics of bacteria |
9 |
|
|
|
|
pH Exercise |
Handout |
|
09-Jun |
10-Jun |
3 |
Aseptic transfer of microbes (begin) |
5 |
|
|
|
|
Streaking for isolation (begin) |
6 |
|
|
|
|
Cultural characteristics of bacteria |
9 |
|
|
|
|
The Gram stain |
7 |
|
11-Jun |
12-Jun |
|
Aseptic transfer of microbes (finish) |
5 |
|
|
|
|
Streaking for isolation (finish) |
6 |
|
|
|
|
The Gram stain |
7 |
|
16-Jun |
17-Jun |
4 |
Special staining techniques |
8 |
|
|
|
|
Exoenzymes (begin) |
10 |
|
|
|
|
The Gram stain |
7 |
|
18-Jun |
19-Jun |
|
Special staining techniques |
8 |
|
|
|
|
Exoenzymes (finish) |
10 |
|
|
|
|
The Gram stain |
7 |
|
23-Jun |
24-Jun |
5 |
Gram stain practical |
|
|
|
|
|
Internet Studies |
|
|
|
|
|
Protein Concentration |
Handout |
|
|
|
|
Viruses |
Handout |
|
|
|
|
Review for Practical |
|
|
25-Jun |
26-Jun |
|
Mid-term laboratory practical |
1-7 |
|
30-Jun |
01Jul |
6 |
Selective & differential media (begin) |
11 |
|
|
|
|
Further tests for gram negative microorganisms (begin) |
12 |
|
02-Jul |
03Jul |
|
Selective & differential media (finish) |
11 |
|
|
|
|
Further tests for gram negative microorganisms (finish) |
12 |
|
07-Jul |
08-Jul |
7 |
Tests for gram positive cocci (begin) |
13 |
|
|
|
|
DNA Extraction |
Handout |
|
|
|
|
Unknown Assigned |
Handout |
|
09-Jul |
10-Jul |
|
Tests for gram positive cocci (finish) |
13 |
|
|
|
|
pH Indicator Molecules |
Handout |
|
|
|
|
Unknown |
Handout |
|
14-Jul |
15-Jul |
8 |
Miscellaneous chemical tests (begin) |
14 |
|
|
|
|
Unknown |
Handout |
|
16-Jul |
17-Jul |
|
Miscellaneous chemical tests (finish) |
14 |
|
|
|
|
Electrophoresis |
Handout |
|
|
|
|
Unknown |
Handout |
|
21-Jul |
22-Jul |
9 |
Effects of antibiotics (begin) |
15 |
|
|
|
|
Unknown |
Handout |
|
23-Jul |
24-Jul |
|
Effects of antibiotics (finish) |
15 |
|
|
|
|
Unknown |
Handout |
|
28-Jul |
29-Jul |
10 |
Unknown due (no exceptions) |
Handout |
|
|
|
|
Review |
|
|
30-Jul |
31-Jul |
|
Final lab practical |
10-15 |
* All students, faculty and staff must understand that food and drinks cannot be allowed in lab at any time. This includes packaged items in book bags. This is an OSHA violation and can result in significant fines for the school. It is also highly dangerous.
Assigned Reading for Lecture- Atlas
|
WEEK |
TOPIC |
PAGES |
|
7 |
Precipitation Reactions |
106-107 |
|
7 |
Agglutination Reactions |
108-109 |
|
7 |
Enzyme-linked Immunosorbent Assay (ELISA) |
110-111 |
|
7 |
Fluorescent Antibody (FA) Technique |
112 |
|
7 |
Western Blot Technique |
113-114 |
|
5 |
Viruses |
115-116 |
|
5 |
HIV |
117 |
|
5 |
Cytopathic Effects |
118 |
|
4 |
Bacterial Pathogens |
121-142 |
|
4 |
Survey of Fungi |
143-150 |
|
4 |
Parasitic Protozoans & Helminths |
151-175 |
|
2 |
Glycolysis, Fermentation, Krebs Cycle |
177-182 |
LABORATORY PERFORMANCE CRITERIA
1. Lab coats worn during lab work sessions.
2. Microscopes handled, used and stored in a correct manner.
3. Lab tables kept clean and disinfected before and after each lab session.
4. Proficiency in focusing the microscope at all levels.
5. Proficiency in lighting and using a Bunsen burner.
6. Aseptic techniques used when working with cultures.
7. Listening to instructors’ directions and following these directions in the performance of laboratory exercises.
8. Carrying out procedures in a proper, safe and correct manner.
9. Disposal of material in the appropriate manner.
10. Observance of safe laboratory practices.
11. Completion of each laboratory assignment.
12. Working well with partners — Each student should do their part in team activities.
ASSIGNMENTS AND MISSED TESTS:
Students who are absent from a class are responsible for all of the work that is done during that class period and for all assignments made during the missed class period.
One (1) missed lecture exam may be made up if a legitimate excuse is presented to your lecture instructor. All make-ups will be at the convenience of the lecture instructor. Missed laboratory work cannot be made up. This includes quizzes.
The Science Department Chair, Coordinators, and faculty are here to help you. If you are having any problems in your classes, please contact the people who can help you. If we don’t know you are having problems, we can’t help you. Perry Carter, Department Chair, can be reached at 822-3443; Dr. Regina Hoffman, Airport Coordinator, can be reached at
822-3416; Dr. Bert Knesel, Beltline Coordinator, can be reached at 738-7660.
CHANGES:
The instructor reserves the right to make changes in the lecture and laboratory schedule as deemed necessary.
LABORATORY POLICIES AND SAFETY PRECAUTIONS
The laboratory experience is designed to supplement and complement material covered in lecture, and is an integral part of the course. Material may be presented in greater detail in lab than in lecture. The laboratory experience may include dissections, films, demonstrations, experiments, workbook exercises, research and writing assignments, and field trips. The day, time and location of approved field trips will be indicated in the course syllabus. Approved field trips will be held during regularly scheduled lab times. Students will provide their own transportation. The laboratory instructors will give a sufficient amount of information and guidance to allow students to complete each lab, but success in lab primarily involves the individual effort of each student. To maximize learning and to minimize the risk of accidents or injury, the following policies and rules will be observed in science labs:
1. Laboratory exercises must be completed during the assigned lab period unless otherwise designated by your instructor.
2. Students are expected to read laboratory exercises before coming to class.
3. Do not disturb any equipment or demonstrations that have been set up, until your instructor has told you how to proceed with
the assignment.
4. Apply yourself in lab. The exercises are designed to reinforce and expand upon material presented in the lecture. Follow
directions carefully.
5. No more than four people can work at any laboratory bench at the same time.
6. Do not hesitate to ask your lab instructor for clarification of any instructions you do not understand. However, do not expect
your lab instructor to simply give you answers to information you should be obtaining through the completion of the various lab
exercises.
7. Bring all necessary materials to lab, including your text.
8. Do not bring any food or drinks into any science lab.
9. Know where the eyewash station and shower are located in each lab. Report injuries immediately to your lab instructor.
10. Protective eyewear is not needed in microbiology, but lab coats are ESSENTIAL.
11. At the end of each lab, leave your work space in good order by discarding waste materials, cleaning and disinfecting your lab
table, returning all materials used to their proper place, and sliding your chair back under the table. Wash your hands.
12. No visitors are allowed in lab or lecture.
13. Neglect of proper safety procedures will result in the student being required to leave the lab and will be required to meet with
the department chair before re-admittance.
MIDLANDS TECHNICAL COLLEGE SCIENCE DEPARTMENT
CODE OF CONDUCT
Student rights and responsibilities are outlined in the Student Handbook. We are extremely proud of the quality of students in the Science Department, however, there have been occasions where disciplinary action is necessary to prevent disruptive and dishonest behavior. The following items are specific violations and consequences supported by the Science Department. Your instructor will circulate a form for your signature stating that you understand the Science Department Course Syllabus, which includes this document.
1. Any student who exhibits behavior that is disruptive to the learning process such as talking, discourtesy to faculty or fellow students to include obscene language or gestures, or uncooperative actions will be asked to leave the classroom. The student will be counted absent for this class. Depending upon the nature of the offense or if it occurs during an exam the instructor may require that the student see the Science Coordinator, Chair of the Science Department, or the Director of Campus Life before returning to class. Campus Security will be called for any threatening or violent behavior.
2. Beepers, cell phones, personal stereos, and similar devices are not permitted in class. Permission must be obtained
from the Science Coordinator or Instructor for students who are emergency personnel or where there are extenuating
circumstances. Campus Security can locate a student and will interrupt a class if there is a situation that needs
immediate attention.
3. Any student proven to have engaged in academic dishonesty will be given a grade of zero on the exam or assignment.
This includes, but is not limited to, giving or receiving information during an exam, use of unauthorized materials
during an exam or assignment, plagiarism, or changing answers after a grade has been assigned. An instructor must
have reasonable proof that dishonesty has occurred. Until an incident is verified, the student will be assigned a grade of “I” for the work. Witnesses of cheating should report this immediately to the instructor. The grade will be discussed confidentially with the student. If the student denies that academic dishonesty occurred, the Chair of the Science Department or Science Coordinator will meet with the instructor and student. The instructor will be supported if departmental guidelines for handling cheating incidences were followed. However, the student is referred to the Student Handbook for the policy on filing a grievance. In any incident involving academic dishonesty, a report will be filed with the Director of Campus Life.
4. Students with complaints about instructors should follow the appropriate chain of command as outlined in the "Science Department Conflict Resolution" form. A form can be obtained from the Science Department. Signatures must be obtained at each level before the complaint will be validated. There may be some circumstances where the first contact is with the Science Coordinator who will discuss the problem with the instructor. All efforts possible will be made to resolve conflicts internally. However students should remember that matters could also be handled through the Academic Appeal/Grievance process detailed in the Student Handbook.