Biology 101 Study Guide for Lab Quiz #2

CELL, DIFFUSION & OSMOSIS, & MITOSIS

The student will be able to define

· Cytology

· organelles

The student will be able to define and give the specific examples observed in lab:

· Symbol indicates specific examples

* Indicates student needs to recognize slide under microscope

Prokaryotic cell

· Cyanobacteria

·      Oscillatoria and Goeocapsa

· Bacteria

·            Lactobacillus

·       Eukaryotic cell

· Protist (unicellular)

·      Amoeba*

·      Paramecium*

Plant cells

·      Elodea

·      Potato

·      Onion

· Animal cells

·       Human epithelial cell

The student will identify the parts of a plant cell from models or diagrams and give a general function:

·       Plasma membrane

·       Cell wall (contains cellulose)

·       Central vacuole

·       Cytoplasm

·       Endoplasmic reticulum (SER and RER)

·       Cytoplast*

· Stroma

· Thylakoid

· Granum

·       Plasmodesmata

·       Mitochondrion

·       Nucleus & Nuclear membrane (envelope)

·       Nucleolus

·       Plastid

·       Amyloplasts

The student will identify the parts of an animal cell from models or diagrams and give a general function. The symbol * indicates item should be recognized from a slide in addition to model or photo

·       Plasma membrane

·       Cytoplasm

·    Endoplasmic reticulum (rough and smooth) RER & SER Ribosome

·    Mitochondria

·    cristae

·    Golgi apparatus

·    Lysosome

·    Centrioles

·    Cilia* - from microscope slide of paramecium

·    Nucleus*

·    Nuclear membrane (envelope)

·    Nucleolus

·    Chromatin

The student will be able to compare the shapes of amoeba and paramecium.

The student will know the general procedure for preparing a wet mount and will know the stain used with each structure/cell:

·   0.1% neutral red stain for onion cell nucleus

·   Janus Green B and 7% sucrose for mitochondria in onion

·   Iodine stain for Amyloplasts (plastids that store starch) in potato

·   Methylene blue for human epithelial (cheek) cells

The student will define:

·   Brownian movement

·   Diffusion

·   Selective permeability

·   Solution

·   Solute

·       Solvent

The student will describe what happens to a cell placed in the following solutions:

·   Hypotonic

·   Hypertonic

·   Isotonic

The student will identify the stages of the cell cycle on the starfish mitosis slides or Allium root slide and cell models (Be able to place models in the correct order):

·   Cytokinesis

·   Interphase

·   Mitosis:  Prophase, Metaphase, Anaphase, Telophase

The student will define the role of each of the following in the cell cycle:

·        Centromere

·        Mitotic spindle

·        Cleavage furrow

·        Cell plate