LAB QUIZ #3

ENZYMES, PHOTOSYNYTHESIS, AND RESPIRATION

The student will describe how an enzyme works and be able to describe and label

·     active site of an enzyme

·     substrate

·     enzyme-substrate complex

·     product

The student will understand how each of the following may affect enzyme activity:

·     energy of activation

·     effect of concentration of substrate

·     effect of pH

·     effect of temperature

·     denaturation

The student will describe the reaction and how each enzyme was used in lab exercises:

·     rennin

·     catalase

·     pectinase

The student will define

             · photosynthesis

                          light & clorophyll

                        equation= 6C0₂ + 6H₂0--------------C₆H₁₂0₆ + 60₂

know each component of the equation for photosynthesis

The student will know the major contributions in

·     light-dependent reactions

splits water to release oxygen, electrons, protons

·     light-independent reactions

fixes carbon dioxide to sugar

The student will understand paper chromatography technique

·     Separates pigments

·       Identify the pigments

·       Yellow — xanthophylls

·       Yellow-orange — carotenes

·       Blue-green — chlorophyll a

·       Yellow green — chlorophyll b

·     Identify the plant source used in lab

·     Identify the solvent

·     What determines how the pigments separate?

The student will explain how you could determine if starch is being produced by leaves by describing the steps in the leaf experiment:

               ·    Purpose of boiling in water

               ·    Purpose of boiling in alcohol

               ·    Iodine staining

The student will explain cellular respiration by describing

·        Aerobic

·        Anaerobic

·        ATP

·        Glycolysis

·        Citric acid cycle (Kreb’s cycle)

·        Electron transport chain

·        Aerobic Respiration:  Equation = C₆H₁₂0₆ + 602 —*6C0₂ + 6H₂0 + ATP + heat

·          Anaerobic Respiration in Plants and some microbes (fermentation)

·          C₆H₁₂0₆ ~ 2 Ethanol+ 2 CO₂ + ATP + heat

·        Anaerobic Respiration in Animals and some microbes (fermentation) C₆H₁₂0₆ -* 2 Lactic acid + ATP + heat

The student will understand how a respirometer was used to measure cellular respiration and describe how each were used or measured in the experiment:

·   Yeast

·   Sugars

·   Carbon dioxide