Study Guide for Lab Quiz #1

 

SCIENTIFIC METHOD, MICROSCOPE & CHEMISTRY

 

The student will know the six steps in using the scientific method.

 

When given an experimental situation the student will be able to identify each step from the following:

 

·    Make observations

·    Develop questions about observations

·    Form hypothesis

·    Set up experimental methods

·    Collect data

·    Conclusions

 

The student will understand the scientific method by defining and giving the importance of:

 

·     Hypothesis

·     Support or reject the hypothesis

·    Control group

·    Experimental group

·    Appropriate conclusions

·    Sample size and replications

·    Statistics

 

The student will be able to identify and use

 

·    Meter stick

·    Beakers and graduated cylinders— giving specific volumes

·    Balance

 

The student will be able to measure using the metric system.

 

·    Length in mm and cm (using a metric ruler)

·    Volume in liter and ml (using a graduated cylinder)

·    Mass in grams (using a balance)

·    Temperature in Celsius (using a thermometer)

·    Density

 

The student will know the parts of the microscope from the microscopes used in lab. The student will know the function and how to use each part:

 

        ·      Ocular lenses (eyepiece) (lOX)

        ·      Arm

        ·      Nosepiece

        ·      Rotating nosepiece

        ·      Objective lenses — identify power of each

        ·      Stage

        ·      Stage (slide) clips

        ·      Mechanical stage

        ·      Fine adjustment

        ·      Coarse adjustment

        ·      Base

        ·      Power switch

        ·      Substage light

        ·      Condenser

        ·    Iris diaphragm lever

 

The student will identify why a given slide is not viewable.

 

The student will identify the proper way to store a microscope.

 

The student will be able to define

        ·     Magnification

        ·     Resolution

 

The student will be able to

 

        ·     Determine the total magnification

        ·     Compare field of view with different objective lens

        ·     Compare and recognize a dissecting and compound microscope

 

Chemistry

 

The student will recognize molecular structure (diagram or model) for the following organic compounds:

       

        ·     Carbohydrate (Glucose and glucose chains)

        ·     Protein (identify an amino acid or chain of amino acid)

        ·     Lipid (identify a fatty acid attached to a glycerol)

        ·     DNA (model)

 

The student will know which compounds:

       

        ·    Are reducing sugars

        ·     Have peptide bonds

        ·     Have nitrogenous bases

        ·     Contains ribose or deoxyribose

        ·     Include monosaccharides, disaccharides, polysaccharides (starch & cellulose)

 

The student will know the difference between a positive control arid a negative control.

 

The student will know which compound is tested with each of the following reagents and will identify the color change with a positive test:

   

        ·     Biuret

        ·     Iodine Benedicts

        ·     Sudan IV

        ·     Dische dephenylamine

   

The student will understand the pH scale and will be able to use indicator paper to determine the pH of given solutions. The student will define:

        ·     Neutral pH

        ·     Acid (Strong and weak)

        ·     Base (Strong and weak)

        ·     Buffer