COURSE SYLLABUS
MIDLANDS
TECHNICAL COLLEGE
SCIENCE
DEPARTMENT
Spring
Semester 2006
COURSE TITLE: MICROBIOLOGY COURSE
NUMBER: BIO 225
CREDIT HOURS: 4.0 LECTURE:
3.0
LABORATORY:
1.0
CONTACT HOURS: 6.0 LECTURE:
3.0
LABORATORY:
3.0
COURSE DESCRIPTION: This is a detailed
study of microbiology as it relates to infection and the disease processes of
the body. Topics include immunity,
epidemiology, medically important microorganisms, and diagnostic procedures for
identification. Application to clinical
health will be emphasized.
COURSE OBJECTIVE: The objective of this course is to assist the student in
gaining a working knowledge of the basic principles and concepts of
microbiology and infectious disease and the application of these concepts to
human disease.
LEARNING OBJECTIVES: Learning objectives are available on the Science
Department Web site or from your instructor either via their website and/or via
handouts.
WEBSITE
ADDRESS:
www.midlandstech.edu/science
REQUIRED TEXT:
Microbiology, An
Introduction, Totorta, 8th, 2003,
Pearson – Benjamin Cummings
MTC Microbiology
Laboratory Manual, Yurko, 1st ed. MTC
Photographic Atlas of
Microbiology Lab, Leboffe, 3rd 99,
Morton
OPTIONAL MATERIAL:
Recommended:
Microbiology Learning Activities, Corbett
and Lema, 1st ed., 2003, Wiley
Recommended: Note Taking
Outline for Microbiology, Corbett and Lema, 1st
ed., 2004, Wiley
Lecture notes or other
material designated by instructor other than required texts listed on this
course syllabus are considered optional.
PREREQUISITES: Bio 101 or Bio 211
DISABILITIES: Students with disabilities requiring in-class
accommodations should call the Counseling/Disabilities Resource Center at
738-7637 (Beltline) or 822-3505 (Airport).
ATTENDANCE POLICY: Students are expected to be present
and ready to work when class begins. Students are expected to attend every
class possible. Cuts should be carefully used since there is no such
thing as an "excused absence". Students will be allowed to miss twice the
number of times a lecture or laboratory section meets per week.
If the lecture meets 3
times per week, 6 absences are allowed.
If the lecture meets 2
times per week, 4 absences are allowed.
If the laboratory meets
2 times per week, 4 absences are allowed.
If the student misses
more than 10 minutes of class by either arriving late or leaving early, then
the student will be counted as absent, missing fewer than 10 minutes is a
tardy. Three tardies count as one
absence.
Students are not to go
in and out of the class during lecture or an exam unless there is an
emergency. Students leaving the
classroom will be counted tardy for missing any time or absent for missing more
than 10 minutes of class time. Students should make arrangements
to go to the restroom between classes, not during class.
Students adding courses
after classes begin are responsible for work covered from the first day of
classes. All classes missed are counted as absences.
When a student exceeds
the maximum allowable absences, a grade of "W" will be assigned if
the student is passing the course or "WF" if the student is failing
after midterm. A "WF" is
calculated into the grade point average as an "F.”
ASSIGNMENTS AND MISSED
TESTS: Students who are absent
from a class are responsible for all of the work that is done during that class
period and for all assignments made during the missed class period.
One (1) missed lecture
exam may be made up if a legitimate excuse is presented to your lecture
instructor. All make-ups will be at the convenience of the lecture instructor. Documentable evidence of extenuating
circumstances may be required. Missing an examination
because of vacations, trips or appointments (non-emergency) will not be
considered an extenuating circumstance. Make-up exams may be written
(objective, discussion), oral or a combination and may differ significantly in
format from those administered at the regular examination period. Students missing the final exam may be
assigned the grade of “0” unless the course instructor has received
notification within 24 hours following the examination. At the instructors discretion students who
contact the instructor prior to an exam may be allowed to take that exam at an
alternate time if exceptional reasons exist.
Missed laboratory work
cannot be made up. This includes
quizzes.
Final exams are given
only during the final exam examination period. They may not be given early.
Final exams cannot be exempted or dropped. Final exam grades cannot be curved.
The Science Department
Chair, Coordinators, and faculty are here to help you. If you are having any problems in your
classes, please contact the people who can help you. If we don’t know you are having problems, we can’t help you. The Department Chair Dr. Perry Carter @
822-3443; Dr. Gerry Lopez is Airport Coordinator @ 822-3788; Dr. Bert Knesel is
Beltline Coordinator @738-7660.
EXTRA CREDIT: Extra credit is usually
not available in this course. If extra
credit is offered it must be on a general basis with all students having equal
opportunity to earn these points. Under no circumstances can extra credit be done after the
final examination day.
WITHDRAWAL: If in the event a student wishes to
withdraw from class they are responsible to file the appropriate withdrawal
form. Withdrawal before midterm results in the grade of “W”. Withdrawal
after mid-term will result in the grade of “W” if the course average at
that point in time is 60 or greater. If the course average is less
than 60, a “WF” will be assigned. No withdrawals can be made after the
last day of class.
CHANGES: The instructor reserves the right to make changes in the
lecture and laboratory schedule as deemed necessary.
Additional policies for
the Department are available in the Science Department’s Student Policy and
Procedures Handbook, in the Academic Center, Room 368; Robinson 106; Airport
and Beltline Libraries, and Lindau Engineering Tech. Building, Room 421.
GRADING
GRADE
CALCULATIONS FOR BIO 225
LECTURE GRADE = 75% of the final grade
Exam
1: ____________
Exam
2: ____________
Exam
3: ____________
Exam
4: ____________
Comprehensive Final Exam: ____________
Sum: _____________
Lecture average = (Sum) / 5 (# of lecture exams) = ____________
Lecture points = (lecture average) X 0.75 = ____________
(Each exam counts 15%)
Note: At the instructor’s discretion pop
quizzes may be given at anytime and may count up to 10% of regular exams.
LABORATORY GRADE = 25% of the final grade
Laboratory Practical I (Midterm) = _______ (Possible 325 points)
Laboratory Practical II (Final) = _______ (Possible 325 points)
Microscope Practical ____________ (Possible
40 points)
Stain Practical ____________ (Possible 40
points)
Media Practical ____________ (Possible 40 points)
Identification of a Bacterial Unknown = _______ (Possible 125 points)
Laboratory Technique = _______ (Possible 20 points)
Microbe Reports = _______ (Possible
30 points)
Case studies = _______ (Possible
45 points)
Internet exercise = _______ (Possible 10 points)
Total points = _______ (Possible 1000 points)
Lab grade calculations are based on 1000
points.
Lab average = Total points /1000 = _______
Lab points = (lab average) X 0.25 =
_______
(Lecture points) (Lab points) = (Course Average)
If course average is: Final Course
Grade is:
90-100 A
80-89 B
70-79 C
60-69 D
<60 F
Withdrawal before midterm W
Withdrawal PASSING after midterm W
Withdrawal FAILING (<60) after midterm WF
All course work not completed in the
course will be graded as “0" and counted in the final average. No grades may be dropped in this
course. No extra credit may be given in
this course unless it is equally available to all sections of the course.
LABORATORY GRADING
A.
Pour Plate 5 Points
B.
Aseptic
Transfer 5 Points
C.
Streaking
for isolation 5 Points
D.
Miscellaneous
Exercises 5 Points
TOTAL 20
Points
II. LITERATURE AND INTREPRETATION
A. Microbe Reports 30
Points
Each student will give
2 reports on organisms that will be assigned by the instructor
B. Case Studies 45
Points
C. Internet Exercise 10 Points
A.
Microscope
Practical 40 Points
B.
Staining
Practical 40 Points
C.
Midterm
Practical 325
Points
D.
Media
Practical 40 Points
E.
Final
Practical 325
Points
TOTAL 770 Points
All
laboratory practicals are departmental.
Lab instructors may not drop questions from
practicals, may not curve or scale practicals, and may not give
any bonus credit questions.
IV. IDENTIFICATION OF A BACTERIAL UNKNOWN
A.
Morphology
and Gram Stain 25 Points
1.
Colonial
Morphology (shape, size, margin, elevation, color) - 5 points
2.
Cellular
Morphology (shape, size, arrangement) -
3 points
3.
Gram stain
reaction - 14 points
B.
Identification
of Unknown/Biochemical Test
100 Points
1.
Correct
genus – 25 points
2.
Correct
species – 25 points
3.
Appropriateness
of biochemical tests – 25 points
4.
Correctness
of biochemical test reports – 25 points
TOTAL
125 Points
TOTAL POSSIBLE POINTS: 1000
LAB WORK IN THIS COURSE CANNOT BE MADE
UP. STUDENTS MAY NOT ATTEND LABS OTHER THAN THEIR OWN.
LABORATORY PERFORMANCE
DEFICIENCIES (LPDs)
Students will be
assessed a point penalty (10 points for each violation) for laboratory
deficiencies which are defined as any violation of the performance criteria
such as the following:
Improper disposal of
infectious material
(1)
Swabs placed back in paper packaging before discarding
(2)
Swabs placed anywhere other than discard bags
(3)
Tubes to be discarded placed anywhere other than discard
test tube racks (such as in the discard bags)
(4)
Plates to be discarded placed anywhere other than discard
bags
Inability to properly
light and use the Bunsen burner
(1)
Failure to adjust the Bunsen burner properly
(2)
Cutting off the Bunsen burner at the needle valve (lowest
valve on the burner)
(3)
Failure to cut off the Bunsen burner at the jet valve (gas
faucet) on the lab table
Improper storage and use
of the microscope
(1)
Placing the microscope in backwards (oculars always face
out)
(2)
Any lens other than the lowest power left down
(3)
Stage left up
(4)
Oil left on the microscope
(5)
Oil used on any lens other than the oil immersion lens
(6)
Dimmer (rheostat) left on
(7)
Switch left on
Failure to wear lab
coats at all lab work sessions
**Students who leave lab without turning off their Bunsen
burner, who ignite, melt or damage any fixtures or instruments in any way or
who do harm to any person in lab shall be dismissed from lab and required to
meet with the department chairman before returning to lab. Absences will be
accrued during that time. A 50 points deduction will be made from the final
summation of lab points.
Where to put it.
|
Culture tubes |
Discard racks |
|
Reagent ampules |
Biohazard bags |
|
Plate cultures |
Biohazard bags |
|
Slides |
Biohazard bags |
|
Contaminated material
(other than tubes) |
Biohazard bags |
|
Paper (includes swab
packaging) |
Trash can |
|
Swabs |
Biohazard bags |
WRITTEN WORK
All written work done by
students in partial fulfillment of the course requirements must be typed. This includes organism reports and class
assignments and/or case study reports.
Instructors may not accept hand written reports. All MTC students have access to our computer
labs and it is required that all students be computer literate.
All written work in
lecture and/or lab must bare the student’s name, which normally should be
placed in the upper right hand corner. No name – no grade.
Written work may not be
turned in binders or folders. Loose pages may not be accepted.
Spelling does count and
may be graded.
Unknown morphology and
unknown report forms are expected to be hand written since they are done in
lab. However, how well the information is reported by the student may be
considered in grading. Neatness and completeness do count.
Work not turned in on
time will be accessed a 10% point penalty per incidence. Faulty may refuse to
accept work that is more than one week later than the deadline.
LABORATORY PERFORMANCE
CRITERIA
1. Lab coats worn
during lab work sessions.
2. Microscopes
handled, used and stored in a correct manner.
3. Lab tables kept
clean and disinfected before and after each lab session.
4. Proficiency in
focusing the microscope at all levels.
5. Proficiency in
lighting and using a Bunsen burner.
6. Aseptic
techniques used when working with cultures.
7. Listening to instructors’ directions and following these directions in the performance of laboratory
exercises.
8. Carrying out
procedures in a proper, safe and correct manner.
9. Disposal of
material in the appropriate manner.
10. Observance of
safe laboratory practices.
11. Completion of
each laboratory assignment.
12.
Working well with partners — Each student should do their
part in team activities.
LABORATORY
POLICIES AND SAFETY PRECAUTIONS
The laboratory
experience is designed to supplement and complement material covered in
lecture, and is an integral part of the course. Material may be presented in greater detail in lab than in
lecture. The laboratory experience may
include dissections, films, demonstrations, experiments, workbook exercises,
research and writing assignments, and field trips. The day, time and location of approved field trips will be
indicated in the course syllabus.
Approved field trips will be held during regularly scheduled lab
times. Students will provide their own
transportation. The laboratory
instructors will give a sufficient amount of information and guidance to allow
students to complete each lab, but success in lab primarily involves the
individual effort of each student. To
maximize learning and to minimize the risk of accidents or injury, the
following policies and rules will be observed in science labs:
1. Laboratory exercises must be completed during the assigned lab period unless otherwise designated by your
instructor.
2. Students are expected to read laboratory
exercises before coming to class.
3. Do not disturb any equipment or demonstrations that have been set up, until your instructor has told you how
to proceed with the assignment.
4. Apply yourself in lab. The exercises are designed to reinforce and expand upon material presented in the
lecture. Follow directions carefully.
5. No more than four people can work at any
laboratory bench at the same time.
6. Do not hesitate to ask your lab instructor for clarification of any instructions you do not understand. However,
do not expect your lab instructor to simply give you answers to information you should be obtaining through
the
completion of the various lab exercises.
7. Bring all necessary materials to lab,
including your text.
8. Do not bring any food or drinks into any
science lab.
9. Know where the eyewash station and shower are located in each lab. Report injuries immediately to your lab
instructor.
10. Protective
eyewear is not needed in microbiology, but lab coats are ESSENTIAL.
11. At the end of each lab, leave your work space in good order by discarding waste materials, cleaning and
disinfecting your lab table, returning all materials used to their proper place, and sliding your chair back under
the table. Wash your hands.
12. No visitors are allowed
in lab or lecture.
13. Neglect of proper safety procedures will result in the student being required to leave the lab and will be required
to meet with the department chair before re-admittance.
MIDLANDS TECHNICAL COLLEGE SCIENCE DEPARTMENT
CODE OF CONDUCT
Student rights and responsibilities are outlined in the
Student Handbook. We are extremely proud of the quality of students in the
Science Department; however, there have been occasions where disciplinary
action is necessary to prevent disruptive and dishonest behavior. The following items are specific violations
and consequences supported by the Science Department. Your instructor will circulate a form for your signature stating
that you understand the Science Department Course Syllabus, which includes this
document.
2. Beepers, cell
phones, personal stereos, and similar devices are not permitted in class.
Permission must be obtained from the Science Coordinator or Instructor for
students who are emergency personnel or where there are extenuating
circumstances. Campus Security can locate
a student and will interrupt a class if there is a situation that needs
immediate attention.
3.
Any student proven to have engaged in academic dishonesty
will be given a grade of zero on the exam or assignment. This includes, but is
not limited to, giving or receiving information during an exam, use of
unauthorized materials during an exam assignment, plagiarism, or changing
answers after a grade has been assigned.
An instructor must have reasonable proof that dishonesty has occurred. Until an incident is verified, the student
will be assigned a grade of “I” for the work.
Witnesses of cheating should report this immediately to the
instructor. The grade will be discussed
confidentially with the student. If the student denies that academic dishonesty
has occurred, the Chair of the Science Department or Science Coordinator will
meet with the instructor and student.
The instructor will be supported if departmental guidelines for handling cheating incidences were
followed. However, the student is
referred to the Student Handbook for the policy on filing a grievance. In any incident involving academic
dishonesty, a report will be filed with the Director of Campus Life.
4. Students with
complaints about instructors should follow the appropriate chain of command as outlined
in the "Science Department Conflict Resolution" form. A form can be obtained from the Science
Department. Signatures must be obtained
at each level before the complaint will be validated. There may be some circumstances where the first contact is with
the Science Coordinator who will discuss the problem with the instructor. All
efforts possible will be made to resolve conflicts internally. However students should remember that
matters can also be handled through the Academic Appeal/Grievance process
detailed in the Student Handbook.
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Week |
Lecture
Topic |
Assigned
Reading |
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Textbook |
Atlas |
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1 |
Introduction |
Chapter 1 |
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Chemical
Principles (Review Only) |
Chapter 2 |
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Microscopy
(Covered in Lab) |
Chapter 3 |
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Prokaryotic
Cell |
Chapter 4 |
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2 |
Microbial
Metabolism |
Chapter 5 |
pp201-208 |
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Microbial
Growth |
Chapter 6 |
pp83-88 |
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3 |
Control
of Microbial Growth |
Chapter 7 |
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Examination
I (Chapters 1,2,4,5,6,7) |
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4 |
Microbial
Genetics |
Chapter 8 |
pp109-112 |
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Biotechnology |
Chapter 9 |
p107,108 |
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5 |
Classification |
Chapter 10 |
p107,108 |
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Prokaryotic
Microbes: Bacteria |
Chapter 11 |
pp129-158 |
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6 |
Eukaryotic
Microbes: Fungi, Algae |
Chapter 12 |
pp159-173 |
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Eukaryotic
Microbes: Protozoans, Helminths |
Chapter 12 |
pp175-200 |
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7 |
Acellular
Microbes: Viruses |
Chapter 13 |
pp123-128 |
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Examination
II (Chapters 8,9,10,11,12) |
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8 |
Principles
of Disease and Epidemiology |
Chapter 14 |
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Mechanisms
of Pathogenicity |
Chapter 15 |
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9 |
Nonspecific
Defenses |
Chapter 16 |
pp99-104 |
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10 |
The
Immune Response |
Chapter 17 |
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The
Immune Response |
Chapter 17 |
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11 |
Applications
of Immunology |
Chapter 18 |
pp113-121 |
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Immune
Disorders |
Chapter 19 |
p124 |
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12 |
Antimicrobial
Chemotherapy |
Chapter 20 |
pp89-90 |
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Examination
III (Chapters 14,15,16,17,18,19,20) |
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13 |
Disease:
Skin, Eyes, Nervous System |
Chapters 21,22 |
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Disease:
Circulatory, Lymphatic, Respiratory |
Chapters 23,24 |
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14 |
Disease:
Digestive, Urinary, Reproductive |
Chapters 25,26 |
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Examination
IV (Chapters 21,22,23,24,24,26) |
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TBA |
Departmental
Comprehensive Final Examination |
Chapters 1-26 |
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Final
Exams cannot be given early. |
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Date |
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Week |
Lab
Topic |
Assigned
Reading |
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Module |
Textbook |
Atlas |
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11-Jan |
12-Jan |
1A |
Safety
and Introduction to the Microbiology Lab |
1 |
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Microscopy
(Begin) |
1 |
pp54-67 |
pp23-26 |
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16-Jan |
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No Labs |
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17-Jan |
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No Labs -
Lecture meets as usual |
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18-Jan |
19-Jan |
1B |
Microscopy
(Continued) |
1 |
pp54-67 |
pp23-26 |
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23-Jan |
24-Jan |
2A |
Microscopy
(Continued) |
1 |
pp54-67 |
pp23-26 |
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Oil
immersion |
1 |
pp54-67 |
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25-Jan |
26-Jan |
2B |
Microscopy
(Finish) |
1 |
pp54-67 |
pp23-26 |
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Wet Mount
(Begin) |
2 |
pp54-67 |
pp43-44 |
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30-Jan |
31-Jan |
3A |
Wet Mount
(Finish) |
2 |
pp54-67 |
pp43-44 |
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Pour
plate (Begin) |
3 |
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Ubiquity
(Begin) |
4 |
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Yeast
Exercise (Handout) |
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01-Feb |
02-Feb |
3B |
Pour
plate (Finish) |
3 |
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Ubiquity
(Finish) |
4 |
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Cultural
characteristics (Begin) |
9 |
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pp1-12 |
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Microscope
Practical |
1&2 |
pp54-67 |
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06-Feb |
07-Feb |
4A |
Aseptic
Transfer (Begin) |
5 |
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Streaking
for isolation (Begin) |
6 |
p169 |
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Cultural
characteristics (Continued) |
9 |
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08-Feb |
09-Feb |
4B |
Aseptic
Transfer (Finish) |
5 |
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Streaking
for isolation (Finish) |
6 |
p169 |
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Isolation
quiz plate (Begin) |
6 |
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Cultural
characteristics (Continued) |
9 |
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pp1-12 |
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Simple
stain (Handout) |
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p67 |
p27 |
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13-Feb |
14-Feb |
5A |
Isolation
quiz plate (Finish) |
6 |
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Cultural
characteristics (Continued) |
9 |
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pp1-12 |
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15-Feb |
16-Feb |
5B |
The Gram
Stain (Continued) |
7 |
pp67-69 |
pp35-38 |
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Capsule
stain (Handout) |
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pp69-71 |
pp1-12 |
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20-Feb |
21-Feb |
6A |
The Gram
Stain (Continued) |
7 |
pp67-69 |
pp35-38 |
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Special
staining techniques (Begin) |
8 |
pp69-71 |
pp38-42 |
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Cultural
characteristics (Continued) |
9 |
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Exoenzymes
(Begin) |
10 |
|
pp75,55,62 |
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22-Feb |
23-Feb |
6B |
The Gram
Stain (Finish) |
7 |
pp67-69 |
pp35-38 |
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Exoenzymes
(Finish) |
10 |
|
pp75,55,62 |
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Cultural
characteristics (Finish) |
9 |
|
pp1-12 |
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pH
Exercise (Handout) |
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pp36-37 |
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27-Feb |
28-Feb |
7A |
Staining
Practical |
7&8 |
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Review
for Midterm Laboratory Practical |
1-9 |
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1-Mar |
2-Mar |
7B |
Midterm
Laboratory Practical |
1-9 |
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06-Mar |
07-Mar |
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No Labs |
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08-Mar |
09-Mar |
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No Labs |
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13-Mar |
14-Mar |
8A |
Selective
& differential media (Begin) |
11 |
pp167-168 |
pp16,18 |
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|
15-Mar |
16-Mar |
8B |
Selective
& differential media (Finish) |
11 |
pp167-168 |
pp16,18 |
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|
|
|
|
pH
Indicator Molecules (Handout) |
|
|
|
|||
|
20-Mar |
21-Mar |
9A |
Test for
Gram-negative microorganisms (Begin) |
12 |
|
pp60,67,80,51,78,79 |
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|
|
|
9B |
Test for
Gram-negative microorganisms (Finish) |
12 |
|
pp60,67,80,51,78,79 |
|||
|
|
|
|
Electrophoresis |
|
pp106-107 |
|
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|
27-Mar |
28-Mar |
10A |
Tests for
Gram-positive cocci (Begin) |
13 |
|
pp50,48,52,71,46 |
|||
|
|
|
|
Unknown
assigned (Handout) |
|
|
|
|||
|
29-Mar |
30-Mar |
10B |
Tests for
Gram-positive cocci (Finish) |
13 |
|
pp50,48,52,71,46 |
|||
|
|
|
|
Unknown
continued (Handout) |
|
|
|
|||
|
03-Apr |
04-Apr |
11A |
Miscellaneous
chemical tests (Begin) |
14 |
|
pp57-58,68-69,72,64 |
|||
|
|
|
|
Case
studies (Begin) (Handout) |
|
|
|
|||
|
|
|
|
Unknown
continued (Handout) |
|
|
|
|||
|
05-Apr |
06-Apr |
11B |
Miscellaneous
chemical tests (Finish) |
14 |
|
pp57-58,68-69,72,64 |
|||
|
|
|
|
Unknown
continued (Handout) |
|
|
|
|||
|
10-Apr |
11-Apr |
12A |
Effects
of antibiotics (Begin) |
15 |
|
pp89-90 |
|||
|
|
|
|
Unknown
continued (Handout) |
|
|
|
|||
|
12-Apr |
13-Apr |
12B |
Effects
of antibiotics (Finish) |
15 |
|
pp89-90 |
|||
|
|
|
|
Unknown
continued (Handout) |
|
|
|
|||
|
17-Apr |
18-Apr |
13A |
DNA
Extraction (Handout) |
|
pp105-106 |
|
|||
|
|
|
|
Media
Practical |
10-14 |
|
|
|||
|
|
|
|
Unknown
continued (Handout) |
|
|
|
|||
|
19-Apr |
20-Apr |
13B |
Protein
concentration (Handout) |
|
|
|
|||
|
|
|
|
Internet
studies (Handout) |
|
|
|
|||
|
24-Apr |
25-Apr |
14A |
Unknown
due (No exceptions) |
|
|
|
|||
|
|
|
|
Review
for Final Laboratory Practical |
10-15 |
|
|
|||
|
|
|
|
Internet
studies due |
|
|
|
|||
|
26-Apr |
27-Apr |
14B |
Final
Laboratory Practical |
10-15 |
|
|
|||
|
|
|
|
Case
studies due |
|
|
|
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